Antioxidant and Antibacterial Potential of Murraya Konigii against Human Cariogenic Pathogens

This study was to evaluate the antioxidant and antibacterial efficacy of herbal crude extract of Murraya koenigii against human Cariogenic pathogens. In this study Methanolic extract of Murraya koenigii were studied for phytochemical composition and GC-MS for active compound. Antioxidant potential assay by 1,1-Diphenyl2-picrylhydrazyl (DPPH), 2, 2’-azinobis-3 ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assays. Further extracts were studied for antibacterial activity. Methanolic extract of Murraya koenigii revealed the presence of terpenoids, alkaloids, tannins, steroids, and saponins. Reducing ability of extracts was in the range (in μm Fe(II)/g) of 3176.98±63.87 were closed to quercetin 3208.27 ± 31.29. A significant inhibitory effect of extract (IC50 = 0.019 ± 0.69 mg/ml) on ABTS free radicals was detected. The antioxidant activity of the extract (96%) DPPH radicals were comparable with that of ascorbic acid (98.8%) and BHT (97.5%). Antibacterial effect of 50% and 10% methanolic extracts was detected on Cariogenic pathogens as compared with standard drug Amoxicillin. GC-MS results revealed the presence of hexadecanoic acid, linoleic acid, oleic acid, propanoic acid, acetophenone. Extracts of Murraya koenigii contain significant amounts of phytochemicals, antioxidant and antibacterial property and it is exploited as a potential source for natural medicine. INTRODUCTION: Dental caries is an infectious microbial disease that results in localized dissolution and destruction of the calcified tissues of the teeth . The untreated condition may lead to pain, tooth loss, infection and finally death in severe cases. Today, caries remains one of the most common diseases throughout the world. Streptococcus mutans is known as the causative bacteria in the formation of dental plaque and dental caries. The acid producing S. mutans causes damage by dissolving tooth structures in the presence of fermentable carbohydrates such as sucrose, fructose, and glucose . The food debris, acid, bacteria, and saliva combine in the mouth to form a sticky substance called “plaque” that adheres to the teeth . Dental disease is painful, and most importantly, it has also been suggestively linked to diabetes, high blood pressure, heart disease. The pain can be worsened by heat, cold or sweet foods and drinks 4, . Treatment often prevents further infection of the tooth structure. Early treatment is less painful than treatment of extensive decay. Dental caries can also cause bad breath and foul tastes. In highly progressed cases, infection can spread from the tooth to surrounding soft tissues which may lead to an